HIV Type 1 p24 Antigen ELISA 2.0 (5 X 96 Determinations)
RETRO-TEK HIV-1 p24 Antigen ELISA 2.0 Kits can also be used to determine the titer of HIV-1 based lentiviral samples. Since the amino acid sequence of p24 is well conserved among the various HIV-1 isolates, this assay detects p24 from HIV-1 clades A-F. There is no cross reactivity to Human Immunodeficiency Virus Type-2 (HIV-2), Simian Immunodeficiency Virus (SIV) or Human T-Cell Leukemia Virus Types I and II (HTLV I and II) p24, or any gag gene product.
Microplate wells are coated with a monoclonal antibody specific for the p24 gag gene product of HIV-1. HIV-1 p24 antigen in the sample is specifically captured onto the immobilized antibody during sample incubation. The captured antigen is then reacted with a human anti-HIV-1 antibody conjugated to horseradish peroxidase (HRP). Following the subsequent addition of substrate, color develops as the HRP enzyme reacts. The resultant optical density is proportional to the amount of HIV-1 p24 antigen present in the sample. The absorbance values of a set of standard dilutions are then plotted. The amount of p24 is determined by interpolation from a point-to-point plot or from a linear regression analysis of the standard curve.
Kit comes with five HIV-1 p24 antibody coated microplates for 96 determinations (per plate), 5 x 12 mL HIV-1 p24 detector antibody, 5 x 0.5 mL HIV-1 p24 antigen standards, 5 x 5 mL lysing buffers, 5 x 100 mL assay diluent, 5 x 125 mL 10x plate wash buffers, 5 x 12 mL substrate, 5 x 12 mL stop solutions, 50 plate sealers, and five resealable plastic bags.
• Wells coated with a monoclonal antibody specific for the p24 gag gene product of HIV-1
• Monitor the purification and biochemical behavior of HIV-1
• Keep refrigerated between 2 to 8°C
• HIV Type 1 p24 Antigen ELISA 2.0 kit
• Determine the titer of HIV-1-based lentiviral samples
• Five 96-well microplates
For Research Use Only. Not for use in Diagnostic Procedures.
For Research Use Only. Not for use in diagnostic procedures. Quality control materials should be used in accordance with local, state, federal, and accreditation requirements. This product is not intended to replace the manufacturer's controls provided with the assay. Propagation or commercialization are prohibited without prior written consent from Antylia Scientific.
The suitability and performance characteristics should be determined by your laboratory for each intended usage.
Use the RETRO-TEK HIV-1 p24 antigen ELISA and ELISA 2.0 immunoassays to detect Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen in research specimens, including cell culture media, as well as human sera and plasma.