HIV Type 1 p24 Antigen ELISA 2.0 (5 X 96 Determinations)

RETRO-TEK HIV-1 p24 Antigen ELISA 2.0 Kits can also be used to determine the titer of HIV-1 based lentiviral samples. Since the amino acid sequence of p24 is well conserved among the various HIV-1 isolates, this assay detects p24 from HIV-1 clades A-F. There is no cross reactivity to Human Immunodeficiency Virus Type-2 (HIV-2), Simian Immunodeficiency Virus (SIV) or Human T-Cell Leukemia Virus Types I and II (HTLV I and II) p24, or any gag gene product.

Microplate wells are coated with a monoclonal antibody specific for the p24 gag gene product of HIV-1. HIV-1 p24 antigen in the sample is specifically captured onto the immobilized antibody during sample incubation. The captured antigen is then reacted with a human anti-HIV-1 antibody conjugated to horseradish peroxidase (HRP). Following the subsequent addition of substrate, color develops as the HRP enzyme reacts. The resultant optical density is proportional to the amount of HIV-1 p24 antigen present in the sample. The absorbance values of a set of standard dilutions are then plotted. The amount of p24 is determined by interpolation from a point-to-point plot or from a linear regression analysis of the standard curve.

Kit comes with five HIV-1 p24 antibody coated microplates for 96 determinations (per plate), 5 x 12 mL HIV-1 p24 detector antibody, 5 x 0.5 mL HIV-1 p24 antigen standards, 5 x 5 mL lysing buffers, 5 x 100 mL assay diluent, 5 x 125 mL 10x plate wash buffers, 5 x 12 mL substrate, 5 x 12 mL stop solutions, 50 plate sealers, and five resealable plastic bags.

• Wells coated with a monoclonal antibody specific for the p24 gag gene product of HIV-1

• Monitor the purification and biochemical behavior of HIV-1

• Keep refrigerated between 2 to 8°C

• HIV Type 1 p24 Antigen ELISA 2.0 kit

• Determine the titer of HIV-1-based lentiviral samples

• Five 96-well microplates