PROtrol SARS-CoV-2 Lineage B.1.617.2; Delta Variant (Isolate: USA/PHC658/2021) (1 mL)

SARS-Related Coronavirus 2 Lineage B.1.617.2; Delta Variant (Isolate: USA/PHC658/2021) is an enveloped, positive-sense single-stranded RNA virus from the Coronaviridae family and the Betacoronaviridae genus. Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/USA/PHC658/2021 was isolated in Memphis, Tennessee, USA in May 2021. Under the nomenclature system introduced by GISAID (Global Initiative on Sharing All Influenza Data), SARS-CoV-2, isolate hCoV-19/USA/PHC658/2021 is assigned lineage B.1.617.2 using Phylogenetic Assignment of Named Global Outbreak LINeages (PANGOLIN) tool. SARS-CoV-2, lineage B.1.617.2 was first detected in India and labelled as a variant of concern (VOC). It was labelled as Delta variant by the World Health Organization (WHO).

SARS-CoV-2 was propagated in VeroE6 cells. The viral culture fluid was clarified via centrifugation to remove cellular debris prior to inactivation.

PROtrol™ (Protein control) is formulated with viral particles that have been modified to render them non-infectious. Each vial contains 1.0 mL of SARS- CoV-2 PROtrol™ frozen in 2% MEM media.

The pre-inactivation titer was determined from an infectious aliquot. Each lot of PROtrol™ is tested using an N-protein ELISA developed internally at ZeptoMetrix. Viral inactivation is verified by the absence of viral growth in tissue culture-based infectivity assay.

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