Description
Each frozen aliquot contains 1 mL of a pure, titered
culture of a recombinant strain of Saccharomyces
cerevisiae. A gene specific to Pneumocystis
jiroveci was inserted as a single copy into the
genome of S. cerevisiae using standard
recombinant techniques. S. cerevisiae was
confirmed by rDNA sequencing. The insert was
detected with a specific in-house real-time PCR
assay. The purity of the culture was monitored by
additional culturing and Gram staining to detect
any contaminating bacteria. The titer was
performed on a minimum of two aliquots after
freezing. The freezing medium contains 15%
glycerol as a cryoprotectant. Please see the
Certificate of Analysis for the specific freezing
medium used.
Intended Use
Saccharomyces cerevisiae live, titered microorganisms can be used to determine a limit of detection (LOD), in diagnostic assay development or cross-reactivity studies. When used as a control for nucleic acid tests, the same protocols as those used to amplify clinical specimens should be employed.
More Information
P. jiroveci-S.cerevisiae is a biosafety level 1. Please consult your institution’s regulations regarding the use of this organism. For a detailed discussion on biological safety see the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), published by the CDC.