Chlamydia trachomatis Z055; LGVII434; Serovar L2, titered (1 EA) (BSL-3)

Each frozen aliquot contains 0.25 mL of Chlamydia trachomatis that were propagated in a monolayer of L929 cells. The titer is in Chlamydial inclusion forming units (IFU) and is a measure of the infectivity of the material. The inclusions were quantified by direct immunofluorescence. The culture was frozen in sucrose-phosphate-glutamate buffer. This material is partially purified and may contain mouse cells. To maintain viability it is recommended to make any dilution of the C. trachomatis with the diluent buffer provided with the material.

Live, titered microorganisms can be used to determine a limit of detection (LOD), in diagnostic assay development or cross-reactivity studies. When used as a control for nucleic acid tests, the same protocols as those used to amplify clinical specimens should be employed.

Please consult your institution’s regulations regarding the use of LGV serovars of C. trachomatis. Federal guidelines suggest that a BSL-3 facility may be advisable for possession and use of this material. For a detailed discussion on biological safety see the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), published by the CDC.