HIV Type 1 p24 Antigen ELISA (96 Determinations)
Viral antigen in the specimen is specifically captured onto the immobilized antibody during specimen incubation. The captured antigen is then reacted with a high-titered human anti-HIV-1 antibody conjugated with biotin. Following a subsequent incubation with Streptavidin-Peroxidase, color develops as the bound enzyme reacts with the substrate. Resultant optical density is proportional to the amount of HIV-1 p24 antigen present in the specimen.
RETRO-TEK HIV-1 p24 Antigen ELISA Kit comes with one HIV-1 p24 antibody coated microplate for 96 determinations, one vial of lyophilized HIV-1 p24 detector antibody, 25 mL detector antibody diluent, 0.5 mL HIV-1 p24 antigen standard, 5 mL lysing buffer, 0.3 mL Streptavidin-Peroxidase, 100 mL assay diluent, 125 mL 10x plate wash buffer, 0.3 mL substrate, 50 mL substrate buffer, 12 mL stop solution, 10 plate sealers, and one resealable plastic bag.
• Wells coated with a monoclonal antibody specific for the p24 gag gene product of HIV-1
• Monitor the purification and biochemical behavior of HIV-1
• Keep refrigerated between 2 to 8°C
• HIV Type 1 p24 Antigen ELISA kit
• One 96-well microplate
For Research Use Only. Not for use in Diagnostic Procedures.
For Research Use Only. Not for use in diagnostic procedures. Quality control materials should be used in accordance with local, state, federal, and accreditation requirements. This product is not intended to replace the manufacturer's controls provided with the assay. Propagation or commercialization are prohibited without prior written consent from Antylia Scientific.
The suitability and performance characteristics should be determined by your laboratory for each intended usage.
Use the RETRO-TEK HIV-1 p24 antigen ELISA and ELISA 2.0 immunoassays to detect Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen in research specimens, including cell culture media, as well as human sera and plasma.