IMMUNO-TEK™ Rat IgG ELISA (96 Determinations)

Microplate wells coated with polyclonal antibodies to rat IgG form the capture phase of the assay. Rat IgG specimen samples along with the supplied standard are diluted appropriately with Assay Diluent and are then incubated in the microplate wells. After a wash step, captured rat IgG in the well is incubated with detector antibody, a polyclonal anti-rat IgG conjugated to horseradish peroxidase (HRP). This antibody reacts only to the Fc portion of the immunoglobulin molecule allowing for the specific detection of IgG. After another wash step, the chromogenic substrate tetramethyl benzidine (TMB) is added and a blue color develops in proportion to the amount of rat IgG that has been bound to the antibody-coated plate. The enzyme reactionis stopped by the addition of Stop Solution which results in a color change to yellow which can be measured spectrophotometrically at 450 nm. The concentrations of rat IgG are then calculated from a standard curve.

The IMMUNO-TEK Rat IgG ELISA* Kit is a rapid, easy to use enzyme linked immunosorbent assay (ELISA) designed for the measurement of rat IgG in rat serum, plasma, saliva, mucosa, cell culture fluid from rat cells or other biological sample. The assay contains ready-to-use reagents and takes less than two hours to perform. The microplate and detector antibody in the kit react with all subclasses of rat IgG.