Blastomyces dermatitidis-S. cerevisiae Recombinant, titered (1 mL)

Each frozen aliquot contains 1 mL of a pure, titered culture of a recombinant Saccharomyces cerevisiae strain. A Blastomyces dermatitidis-specific gene was inserted as a single copy into the S. cerevisiae genome using standard recombinant techniques. The identity of S. cerevisiae was confirmed by rDNA sequencing, and the insert was detected using an in-house real-time PCR assay. Culture purity was monitored through additional culturing and Gram staining to detect any bacterial contamination. The titer was determined on a minimum of two aliquots after freezing. The freezing medium contains 15% glycerol as a cryoprotectant. See the Certificate of Analysis for details on the specific freezing medium used.

Live, titered microorganisms can be used to determine a limit of detection (LOD), in diagnostic assay development or cross-reactivity studies. When used as a control for nucleic acid tests, the same protocols as those used to amplify clinical specimens should be employed.

Titered material should be stored at -65°C or below.