Pneumocystis jiroveci-S. cerevisiae Recombinant, DNA (1 µg)

Each aliquot contains 1 μg of DNA extracted from a pure culture of a recombinant strain of Saccharomyces cerevisiae. A gene specific to Pneumocystis jirovecii was inserted as a single copy into the genome of S. cerevisiae using standard recombinant techniques. S. cerevisiae was confirmed by rDNA sequencing. The insert was detected with a specific in-house real-time PCR assay. The purity of the culture was monitored by additional culturing and Gram staining to detect any contaminating bacteria. The DNA was extracted from the cells following a protocol based on the yeast protocol provided in the Qiagen® Genomic DNA Handbook and using Qiagen® Genomic DNA Buffers with a 500/G genomic tip. DNA concentration and A260/280 ratios were determined by testing a minimum of two aliquots on a NanoDrop®. The extracted DNA also tested positive on an in- house real time PCR assay.

Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Pneumocystis jirovecii. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross- reactivity studies or genomic sequencing. Controls should be run using the same protocols as those used to amplify extracted clinical specimens.

This control is supplied in TE Buffer and should be frozen at -20°C or below.