ZeptoMetrix
Blastomyces dermatitidis-S. cerevisiae Recombinant, titered (1 mL)
SKU: 0801697
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Description
Each frozen aliquot contains 1 mL of a pure, titered culture of a recombinant strain of Saccharomyces cerevisiae. A gene specific to Blastomyces dermatitidis was inserted as a single copy into the genome of S. cerevisiae using standard recombinant techniques. S. cerevisiae was confirmed by rDNA sequencing. The insert was detected with a specific in-house real-time PCR assay. The purity of the culture was monitored by additional culturing and Gram staining to detect any contaminating bacteria. The titer was performed on a minimum of two aliquots after freezing. The freezing medium contains 15% glycerol as a cryoprotectant. Please see the Certificate of Analysis for the specific freezing medium used.
Intended Use
Live, titered microorganisms can be used to determine a limit of detection (LOD), in diagnostic assay development or cross-reactivity studies. When used as a control for nucleic acid tests, the same protocols as those used to amplify clinical specimens should be employed.
More Information
B. dermatitidis-S. cerevisiae is a biosafety level 1. Please consult your institution’s regulations regarding the use of this organism. For a detailed discussion on biological safety see the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), published by the CDC.
Documents
Product Information
- BrandZeptoMetrix
- Volume1 mL
- Unit of MeasureEA
- Storage Condition-65°C or below
- Product TypeTitered Live Organism
Classification/Permits
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RUO