Coccidioides immitis-S. cerevisiae Recombinant, DNA (1 µg)

Each aliquot contains 1 μg of DNA extracted from a pure culture of a recombinant Saccharomyces cerevisiae strain. A Coccidioides immitis-specific gene was inserted as a single copy into the S. cerevisiae genome using standard recombinant techniques. The identity of S. cerevisiae was confirmed by rDNA sequencing, and the insert was verified using an in-house real-time PCR assay. Culture purity was monitored through additional culturing and Gram staining to detect any bacterial contamination. DNA extraction followed the yeast protocol outlined in the Qiagen® Genomic DNA Handbook, using Qiagen® Genomic DNA Buffers and a 500/G genomic tip. DNA concentration and A260/280 ratios were measured on a NanoDrop® using at least two aliquots. The extracted DNA also tested positive in the in-house real-time PCR assay.

Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Coccidioides immitis. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross- reactivity studies or genomic sequencing. Controls should be run using the same protocols as those used to amplify extracted clinical specimens.

This control is supplied in TE Buffer and should be frozen at -20°C or below.