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ZeptoMetrix

Pneumocystis jiroveci-S. cerevisiae Recombinant, titered (1 mL)

SKU: 0801698

Description

Each frozen aliquot contains 1 mL of a pure, titered culture of a recombinant strain of Saccharomyces cerevisiae. A gene specific to Pneumocystis jiroveci was inserted as a single copy into the genome of S. cerevisiae using standard recombinant techniques. S. cerevisiae was confirmed by rDNA sequencing. The insert was detected with a specific in-house real-time PCR assay. The purity of the culture was monitored by additional culturing and Gram staining to detect any contaminating bacteria. The titer was performed on a minimum of two aliquots after freezing. The freezing medium contains 15% glycerol as a cryoprotectant. Please see the Certificate of Analysis for the specific freezing medium used.

Intended Use

Saccharomyces cerevisiae live, titered microorganisms can be used to determine a limit of detection (LOD), in diagnostic assay development or cross-reactivity studies. When used as a control for nucleic acid tests, the same protocols as those used to amplify clinical specimens should be employed.

More Information

P. jiroveci-S.cerevisiae is a biosafety level 1. Please consult your institution’s regulations regarding the use of this organism. For a detailed discussion on biological safety see the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), published by the CDC.

Product Information

  • Brand ZeptoMetrix
  • Volume 1 mL
  • Unit of Measure EA
  • Storage Condition -65°C or below
  • Product Type Titered Live Organism

Classification/Permits

  • RUO

  • MTA REQUIRED

    Purchase of this live material requires submittal of an executed ZeptoMetrix MTA prior to shipment
  • HAWAII PERMIT

Notifications

  • warning
    This item has been deemed hazardous Additional shipping fees and permits may apply.
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